the shepherd lab
“Science, my boy, is made up of mistakes, but they are mistakes which it is useful to make, because they lead little by little to the truth.” - Jules Verne, Journey to the Center of the Earth
Native human ovarian cancer ascites-derived spheroids have detectable LC3-positive autophagosomal punctae (orange arrowheads).
our research
Autophagy in ovarian cancer spheroids and metastasis: When cells are starved for nutrients they will tend to activate strategies to ensure survival until normal growth conditions are restored. We discovered that ovarian cancer spheroids induce the process of macroautophagy (or commonly referred to as autophagy), a universal process among eukaryotes whereby cells engulf cytoplasmic organelles and macromolecules within double-membraned autophagosomes, then degrade the contents by fusion with lysosomes to recycle essential metabolites under bioenergetic stress conditions. We are currently determining whether autophagy represents a therapeutic vulnerability of ovarian cancer during metastasis and in the state of tumour dormancy during minimal residual disease.